Journal: Cancers

Article Title: Bridging the Species Gap: Morphological and Molecular Comparison of Feline and Human Intestinal Carcinomas

doi: 10.3390/cancers13235941

Figure Lengend Snippet: Four distinct somatic missense mutations were detected, each of them in a different case. DNA forward sequences; green: wildtype sequence of normal intestinal tissue ( n = 3); red: tumor . ( A ) Cytosine (C) is substituted by Thymine (T), resulting in a missense mutation leading to a replacement of Serine (S) by Phenylalanine (F) on the protein level (colonic ANOS; β-catenin score 1); ( B ) Adenosine (A) is substituted by Guanin (G) resulting in a missense mutation leading to a replacement of Aspartic acid (D) by Glycine (G) (colonic ANOS; score 3). For case 20, no normal tissue was available; ( C ) Guanin (G) is substituted by Adenosine (A) resulting in a replacement of Aspartic acid (D) by Asparagine (N) (colonic ANOS, score 1); ( D ) Guanin (G) is substituted by Adenosine (A) resulting in a replacement of Glycine (G) by Arginine (R) (colonic SAC, score 1).

Article Snippet: For capillary electrophoresis the sequencing product was purified using the ZR DNA Sequencing Clean-up Kit TM (Zymo Research Europe GmbH, Freiburg, Germany) and loaded on an ABS/Hitachi 3130 genetic analyzer (Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Sequencing, Mutagenesis